Virus-Induced Gene Silencing in Plants.pdf

Virus-Induced Gene Silencing in Plants

This volume aims at providing a complete and updated overview of gene downregulation in plants performed through virus-induced gene silencing (VIGS). Chapters guide readers through classical and newly developed protocols of VIGS to allow readers to initiate or optimize their own silencing experiments according to the methods. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Virus-Induced Gene Silencing in Plants: Methods and Protocols aims to ensure successful results in the further study of this vital field.

RNA interference (RNAi) is a biological process in which RNA molecules inhibit gene expression or translation, by neutralizing targeted mRNA molecules. Historically, RNAi was known by other names, including co-suppression, post-transcriptional gene silencing (PTGS), and quelling. The detailed study of each of these seemingly different processes elucidated that the identity of these phenomena were all … Post-transcriptional gene silencing in plants | …

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Virus-Induced Gene Silencing in Plants.pdf

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Sofia Voigt

High throughput virus‐induced gene silencing … Holzberg S, Brosio P, Gross C and Pogue GP (2002) Barley stripe mosaic virus‐induced gene silencing in a monocot plant. Plant J, 30, 315 – 327. Wiley Online Library CAS PubMed Web of Science® Google Scholar; Innes RW (1998) Genetic dissection of R gene signal transduction pathways. Curr Opin Plant Biol, 1, 299 – 304.

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Matteo Müller

This volume aims at providing a complete and updated overview of gene downregulation in plants performed through virus-induced gene silencing (VIGS). Chapters guide readers through classical and newly developed protocols of VIGS to allow readers to initiate or optimize their own silencing Efficient Virus-Induced Gene Silencing ... - Plant …

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Noel Schulze

Virus-Induced Gene Silencing in Plant Roots | … Abstract. Virus-induced gene silencing (VIGS) has been used as a quick and easy tool to assess gene function in plants. The virus, carrying a portion of an endogenous gene, triggers a homology-based defense mechanism when introduced into plants.

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Jason Lehmann

Gen-Silencing – Wikipedia

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Jessica Kohmann

Inoculation of Nicotiana benthamiana and tomato seedlings with a mixture of both strains results in gene silencing. Silencing of the endogenous phytoene desaturase ( PDS ) gene, which causes photobleaching, is used as a control for VIGS efficiency. RNA interference - Wikipedia